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At the start of the experiment, Pfa1 cells overexpressing Mock (left) or FSP1 (right) were treated with 250 nM RSL3 to trigger ferroptosis

In this context, it is generally recognised that cancer cells transitioning into the DTP state develop unique vulnerabilities as a consequence of metabolic reprogramming [19]

Angiogenesis Analyzer, Analyze Particles, For the collection of CM, U87MG-GFP, and U87MG-ID1-GFP cells (7.5105) were seeded in a 100-mm culture plate with DMEM containing 10% FBS